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Fifth International Workshop on Arylamine N-acetyltransferases

Paris, September 1 - 3, 2010
Hosted by:

Université Paris Diderot
Campus Paris Rive-Gauche
75013 Paris

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5th NAT WorkShop Participants

Meeting website:


The NAT workshops have been running since 1998. The 5th International Workshop will be hosted by University Paris Diderot. The Workshop will take place from the afternoon of Wednesday September 1 to the morning of Friday September 3, 2010. The 9th International ISSX meeting (Istanbul, Turkey) is scheduled for September 4-8, making it convenient to attend both meetings by flying through Paris.

The Workshop program will consist of topical sessions with invited speakers and contributed presentations in the form of posters. Poster sessions will allow ample time for discussion of research findings.

The program includes the following sessions (Chairs)

  1. Structures and enzymatic functions of NAT (Denis M. Grant, Canada)
  2. NAT in microorganisms (Edith Sim, UK)
  3. NAT polymorphisms, diseases and evolution (Jose A.G. Agundez, Spain)
  4. NAT expression and regulation (Rodney F. Minchin, Australia)
  5. NAT and aromatic amine metabolism (David W. Hein, USA)
  6. NAT nomenclature (Sotiria Boukouvala, Greece; David W. Hein, USA)
  7. Animal models of NAT (Charlene McQueen, USA)

Workshop program:

Symposium A: NAT polymorphisms, diseases and evolution / NAT nomenclature
Chairs: José A.G. Agundez, Spain; David W. Hein, USA; Sotiria Boukouvala, Greece
2:00 pm

 Opening and Welcome Addresses

2:25 pm

 José A.G. Agundez, Badajoz, Spain
Copy number variations in the human NAT1 and NAT2 genes
2:45 pm

 David W. Hein, Louisville, USA
A single nucleotide polymorphism identified in a genome-wide scan tags variation in the N-acetyltransferase 2 phenotype in populations of european background
3:05 pm

 Audrey Sabbagh, Paris, France
Evolutionary pharmacogenetics of the NAT gene family: contrasting histories of the acetylation polymorphism in human and non-human primates
3:25 pm

 Etienne Patin, Paris, France
Deciphering the complex evolutionary history of human arylamine N-acetyltransferase genes
3:45 pm

 Coffee Break

4:15 pm

 Philippe Silar, Paris, France
The evolution of arylamine N-acetyltransferases in fungi
4:35 pm

 Sotiria Boukouvala, Alexandroupolis, Greece
Comparative genomic survey, exon-intron annotation and phylogenetic analysis of NAT homologous sequences in archaea, protists, fungi, viruses and invertebrates
4:55 pm

 Eleni P. Karagianni, Alexandroupolis, Greece
Arylamine N-acetyltransferases in mycotoxigenic and related fungi of agricultural significance
5:15 pm

 David W. Hein, Louisville, USA
Current nomenclature of human arylamine N-acetyltransferases
5:35 pm

 Sotiria Boukouvala, Alexandroupolis, Greece
Current nomenclature of non-human arylamine N-acetyltransferases
5:55 pm

 NAT nomenclature discussion

Symposium B: NAT and aromatic amine metabolism / animal models of NAT
Chairs: David W. Hein, USA; Charlene McQueen, USA
9:05 am

 David W. Hein, Louisville, USA
Associations between N-acetyltransferase 2 (NAT2) single nucleotide polymorphisms (SNPs) and haplotypes with catalytic activity in cryopreserved human hepatocytes: recommendations for NAT2 genotyping
9:25 am

 Peter J. O’Brien, Toronto, Canada
The marked increase in 4-aminobiphenyl induced hepatocyte cytotoxicity using an inflammation/oxidative stress model that causes NAT inhibition
9:45 am

 Denis M. Grant, Toronto, Canada
Roles of DNA damage, mutation rates and inflammation in the gender- and strain- selective liver carcinogenecity of 4-aminobiphenyl in mice
10:05 am

 Mark A. Doll, Louisville, USA
Difference between human slow N-acetyltransferase-2 alleles in levels of 4-aminobiphenyl-induced DNA adducts and mutagenesis
10:25 am

 Coffee Break

10:55 am

 Carmine S. Leggett, Louisville, USA
Construction and functional characterization of nucleotide excision repair-deficient SV40-transformed human fibroblasts expressing human CYP1A2 and NAT2 genetic variants
11:20 am

 Charlene A. McQueen, Auburn, USA
PPAR alpha regulated genes are activated in mice by exposure to hydrazine, a metabolite of isoniazid
12:00 noon


Symposium C: NAT expression and regulation
Chair: Rodney F. Minchin, Australia
2:00 pm

 Keynote lecture: Robert Barouki, Paris, France
The long term cost of detoxication
2:30 pm

 Rodney F. Minchin, Brisbane St Lucia, Australia
Role of human N-acetyltransferase 1 in cell growth and invasion
2:50 pm

 Neville Butcher, Brisbane St Lucia, Australia
Epigenetic regulation of human N-acetyltransferase 1
3:10 pm

 Brunhilde Blömeke, Trier, Germany
NAT1 activity in human keratinocytes and immune cells: possible implications for adaptive immune responses to aromatic diamines
3:30 pm

 Lori M. Millner, Louisville, USA
Differences in arylamine-induced mutagenesis with N-acetyltransferase 1 alternative mRNA isoforms
3:50 pm

 Julien Dairou, Paris, France
Uncoupled reaction of N-acetylation and AcCoA hydrolysis: example of folate
4:10 pm

 Jutta Bonifas, Trier, Germany
Analysis of NAT1 expression and keratinocyte differentiation
4:30 pm

 Florent Busi, Paris, France
Cadmium alters the biotransformation of carcinogenic aromatic amines by arylamine N-acetyltransferases: molecular, cellular and in vivo studies
4:50 pm

 Coffee Break

5:20 pm

 Poster session

Symposium D: Structures and enzymatic functions of NAT
Chair: Denis M. Grant, Canada
9:05 am

 Carston Wagner, Minneapolis, USA
Interactions of arylnitroso metabolites of arylamine carcinogens with NATs in cells
9:25 am

 Fernando Rodrigues-Lima, Paris, France
Crystal structure of Bacillus anthracis arylamine N-acetyltransferase in complex with CoA reveals unusual structural properties
9:45 am

 Nicola Laurieri, Oxford, UK
Optimization of isoenzyme-specific reagents: organic synthesis and biological characterisation of naphtoquinones as selective inhibitors of human N-acetyltransferase 1 (hNAT1) and mouse NAT2 (mNAT2)
10:05 am

 Elodie Sanfins, Paris, France
Effects of carbon black nanoparticles on the biotransformation of carcinogen aromatic amines by the human arylamine N-acetyltransferase 1
10:25 am

 Coffee Break

Symposium E: NAT in microorganisms
Chair: Edith Sim, UK
10:40 am

 Edith Sim, Oxford, UK
A novel reductase in the arylamine N-transferase (nat) operon of Mycobacterium smegmatis
11:00 am

 Xavier Kubiak, Paris France
Molecular characterisation of the arylamine N-acetyltransferase from three clinical strains of Legionella pneumophila
11:20 am

 Areej M. Abuhammad, Oxford, UK
Structural studies of the mycobacterial arylamine N-acetyltransferases
11:40 am

 Jean-Marie Dupret, Paris, France
Arylamine N-acetyltransferases of fungi: a tool for bioremediation of contaminated soils
12:00 noon

 Conclusions and lunch

Beckman Coulter


Dominique Dutscher




Roche diagnostics



Paris Diderot University


© Copyright Florent Busi / Université Paris Diderot, BFA 2010